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HEMATOLOGY |
ITEM / TEST / ASSAY | TEST METHODOLOGY | RUNNING DAY | TURN AROUND TIME | SPECIMEN REQUIREMENT | PATIENT PREPARATION | SPECIAL CONSIDERATIONS |
|
Bleeding Time |
Ivy Method |
Daily |
Routine: Two hours STAT: One hour |
Skin incision using Surgicutt | None |
Take note of patient's medication; apply steri-strip |
|
Bone Marrow Aspirate Smear - Staining ONLY |
Wright's Stain | Daily |
30 minutes |
BMA Smear | None |
This is for staining the specimen only |
|
Cell Count, Cerebrospinal Fluid (CSF) |
Manual Method | Daily |
Routine: Two hours STAT: One hour |
CSF; freshly collected | None |
Must be processed immediately |
|
Cell Count, Other Body Fluids | Manual Method | Daily | Routine: Two hours STAT: One hour |
Other sterile body fluid | None | Must be processed immediately |
|
Clotting Time, Lee and White Method |
Lee and White Method | Daily | Routine: Two hours STAT: One hour |
1cc whole blood (glass red top) | None |
Collected by Medical Technologist only |
|
Clotting Time, Slide Method |
Slide Method | Daily | Routine: Two hours STAT: One hour |
Capillary blood or heel stick | None |
Collected by Medical Technologist only, avoid edematous area |
|
Complete Blood Count with Platelet Count (CBC) |
Flow Cytometry | Daily | Routine: Two hours STAT: One hour |
3cc whole blood / 500uL microtainer (EDTA) | None |
Mix by gentle inversion 8 to10 times |
|
Clot Retraction Time (CRT) |
Manual Method | Daily |
Depends on how long the blood will retract |
1cc whole blood (glass red top) | None |
Take note of the time specimen was collected and place in a water/dry bath 37°C, collected by Medical Technologist only |
|
D-dimer |
Absorbance |
Daily |
Routine: Two (2) hours STAT: One (1) hour |
3cc citrated plasma | None |
Must be processed immediately |
|
Dilute Russell Viper Venom Time (DRVVT) | Absorbance |
Every Wednesday Cut-off – 12:00 NN |
Friday | 3cc citrated plasma | None |
Centrifuge at 3500 rpm for 10 minutes separate plasma in a plastic tube, recentrifuge and transfer plasma into a plastic tube (take care not to include the residual platelets that may have collected at the bottom of the centrifuge tube).. Freeze sample immediately |
|
Fibrinogen | Absorbance | Every Wednesday Cut-off – 12:00 NN |
Four (4) hours | 3cc citrated plasma | None | Centrifuge at 3500 rpm for 10 minutes separate plasma in a plastic tube, recentrifuge and transfer plasma into a plastic tube (take care not to include the residual platelets that may have collected at the bottom of the centrifuge tube).. Freeze sample immediately |
|
Erythrocyte Sedimentation Rate (ESR) |
Westergren Method | Every Wednesday Cut-off – 12:00 NN |
Routine: Two hours STAT: One (1) hour |
2cc whole blood (sodium citrate - black top) | None |
Mix by gentle inversion 8 to10 times |
|
Filaria Blood Film |
Manual Method | Every Wednesday Cut-off – 12:00 NN | Four (4) hours |
3cc whole blood / 500uL microtainer (EDTA)and 2-3 direct smears (Finger prick) |
None |
Collected between 10:00 PM to 2:00 AM (nocturnal blood collection). |
|
Hematocrit |
Electronic Impedance |
Daily |
Routine: Two (2) hours STAT: One (1) hour |
3cc whole blood / 500uL microtainer (EDTA) |
None |
Mix by gentle inversion 8 to10 times |
|
Hemoglobin |
Cyanmet Hemoglobin Spectrophotometry | Daily | Routine: Two (2) hours STAT: One (1) hour | 3cc whole blood / 500uL microtainer (EDTA) | None | Mix by gentle inversion 8 to10 times |
|
Hemoglobin and Hematocrit (H&H) |
See Individual Test | Daily | Routine: Two (2) hours STAT: One (1) hour | 3cc whole blood / 500uL microtainer (EDTA) | None | Mix by gentle inversion 8 to10 times |
|
Le Preparation |
Manual Method | Daily |
Six (6) hours |
Two 6 cc whole blood (red top glass); Do not CENTRI |
None |
None |
|
Malaria Antigen Test |
Test Kit | Daily |
Routine: Two (2) hours STAT: One (1) hour |
3 cc whole blood (EDTA) / 500 uL | None | None |
|
Nasal Smear, Eosinophils |
Manual Method | Daily | Routine: Two (2) hours STAT: One (1) hour | 3 cc whole blood (EDTA) / 500 uL |
Nasal smear | None |
|
Malarial Smear |
Giemsa Stain | Daily |
Six (6) hours |
3 cc whole blood / 500uL microtainer (EDTA) and two to three direct malarial smears (finger prick) | Nasal smear |
Note the travel history of the patient and days of fever |
|
Silica Clotting Time (SCT) |
Absorbance |
Every Wednesday Cut-off: 12:00 NN |
Friday |
3cc citrated plasma |
None |
Centrifuge at 3500 rpm for 10 minutes separate plasma in a plastic tube, re-centrifuge and transfer plasma into a plastic tube (take care not to include the residual platelets that may have collected at the bottom of the centrifuge tube. Freeze sample immediately |
|
Lupus Anticoagulant Test 1 (SCT + DRVVT) |
See Individual Test | Every Wednesday Cut-off: 12:00 NN | Friday | 3cc citrated plasma | None | |
|
Prothrombin Time or Protime (PT) |
Absorbance | Every Wednesday Cut-off: 12:00 NN |
Routine: Two (2) hours STAT: One (1) hour |
3cc citrated plasma | None |
Should be processed within 24 hours/immediately separate citrated plasma in a plastic tube, should there will be a delay in processing. Take note of patient's medication. |
|
PT Mixing Study | Absorbance | Every Wednesday Cut-off: 12:00 NN |
Four (4) hours | 3cc citrated plasma | None |
Must be processed immediately |
|
Partial thromboplastin Time (PTT) Mixing Study | Absorbance | Every Wednesday Cut-off: 12:00 NN | Four (4) hours | 3cc citrated plasma | None | Must be processed immediately |
|
Peripheral Smear |
Manual Method |
Daily |
Four hours |
Freshly collected, must be submitted to hematology within two hours after collection/3cc whole blood / 500uL microtainer (EDTA) or two direct smears (finger prick) |
None |
Mix by gentle inversion 8 to 10 times |
|
Peripheral Smear with Pathologist Consultation | Manual Method | Daily |
Refer to Hematology Section of Pathologist’s Availability | Freshly collected, must be submitted to hematology within two hours after collection/3cc whole blood / 500uL microtainer (EDTA) or two direct smears (finger prick) | None | Mix by gentle inversion 8 to 10 times |
|
Platelet Count Estimation on Smear | Manual Method | Daily |
Routine: Two hours STAT: One hour |
3 cc whole blood / 500uL microtainer (EDTA) | None | Mix by gentle inversion 8 to 10 times |
|
Reticulocyte Count, Manual | Manual Method | Daily | Routine: Two hours STAT: One hour | 3 cc whole blood / 500uL microtainer (EDTA) | None | Mix by gentle inversion 8 to 10 times |
|
Platelet Count |
Flow Cytometry | Daily | Routine: Two hours STAT: One hour | 3 cc whole blood / 500uL microtainer (EDTA) | None | Mix by gentle inversion 8 to 10 times |
|
White Blood Cell (WBC) Count | Flow Cytometry | Daily | Routine: Two hours STAT: One hour | 3 cc whole blood / 500uL microtainer (EDTA) | None | Mix by gentle inversion 8 to 10 times |
|
WBC and Differential Count |
Flow Cytometry |
Daily |
Routine: Two (2) hours STAT: One (1) hour |
3 cc whole blood / 500uL microtainer (EDTA) |
None |
Mix by gentle inversion 8 to 10 times |