HEMATOLOGY SERVICES

HEMATOLOGY

ITEM / TEST / ASSAY
TEST METHODOLOGY
RUNNING DAY
TURN AROUND TIME
SPECIMEN REQUIREMENT
PATIENT PREPARATION
SPECIAL CONSIDERATIONS

Bleeding Time 

Ivy Method 

Daily 

Routine: Two hours

STAT: One hour 

Skin incision using Surgicutt 

None

Take note of patient's medication; apply steri-strip 

Bone Marrow Aspirate Smear - Staining ONLY 

Wright's Stain 

Daily 

30 minutes 

BMA Smear 

None

This is for staining the specimen only 

Cell Count, Cerebrospinal Fluid (CSF) 

Manual Method 

Daily 

Routine: Two hours

STAT: One hour 

CSF; freshly collected 

None

Must be processed immediately 

Cell Count, Other Body Fluids 

Manual Method
Daily 

Routine: Two hours

STAT: One hour 

Other sterile body fluid 

None
Must be processed immediately 

Clotting Time, Lee and White Method 

Lee and White Method 

Daily 

Routine: Two hours

STAT: One hour 

1cc whole blood (glass red top) 

None

Collected by Medical Technologist only 

Clotting Time, Slide Method 

Slide Method 

Daily 

Routine: Two hours

STAT: One hour 

Capillary blood or heel stick 

None

Collected by Medical Technologist only, avoid edematous area 

Complete Blood Count with Platelet Count (CBC) 

Flow Cytometry 

Daily 

Routine: Two hours

STAT: One hour 

3cc whole blood / 500uL microtainer (EDTA) 

None

Mix by gentle inversion 8 to10 times 

Clot Retraction Time (CRT) 

Manual Method 

Daily 

Depends on how long the blood will retract 

1cc whole blood (glass red top) 

None

Take note of the time specimen was collected and place in a water/dry bath 37°C, collected by Medical Technologist only 

D-dimer 

Absorbance 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3cc citrated plasma 

None

Must be processed immediately 

Dilute Russell Viper Venom Time (DRVVT) 

Absorbance 

Every Wednesday Cut-off 12:00 NN 

Friday 

3cc citrated plasma 
None

Centrifuge at 3500 rpm for 10 minutes separate plasma in a plastic tube, recentrifuge and transfer plasma into a plastic tube (take care not to include the residual platelets that may have collected at the bottom of the centrifuge tube).. Freeze sample immediately 

Fibrinogen 

Absorbance 
Every Wednesday Cut-off – 12:00 NN 

Four (4) hours 

3cc citrated plasma 
None
Centrifuge at 3500 rpm for 10 minutes separate plasma in a plastic tube, recentrifuge and transfer plasma into a plastic tube (take care not to include the residual platelets that may have collected at the bottom of the centrifuge tube).. Freeze sample immediately 

Erythrocyte Sedimentation Rate (ESR) 

Westergren Method 

Every Wednesday Cut-off – 12:00 NN 

Routine: Two hours

STAT: One (1) hour 

2cc whole blood (sodium citrate - black top) 

None

Mix by gentle inversion 8 to10 times 

Filaria Blood Film 

Manual Method 

Every Wednesday Cut-off – 12:00 NN 
Four (4) hours 

3cc whole blood / 500uL microtainer (EDTA)and 2-3 direct smears (Finger prick) 

None

Collected between 10:00 PM to 2:00 AM (nocturnal blood collection). 

Hematocrit 

Electronic Impedance 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3cc whole blood / 500uL microtainer (EDTA) 

None 

Mix by gentle inversion 8 to10 times 

Hemoglobin 

Cyanmet Hemoglobin Spectrophotometry 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3cc whole blood / 500uL microtainer (EDTA) 
None 
Mix by gentle inversion 8 to10 times 

Hemoglobin and Hematocrit (H&H) 

See Individual Test 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3cc whole blood / 500uL microtainer (EDTA) 
None 
Mix by gentle inversion 8 to10 times 

Le Preparation 

Manual Method 

Daily 

Six (6) hours 

Two 6 cc whole blood (red top glass); Do not CENTRI 

None 

None 

Malaria Antigen Test 

Test Kit 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3 cc whole blood (EDTA) / 500 uL 

None 
None 

Nasal Smear, Eosinophils 

Manual Method 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3 cc whole blood (EDTA) / 500 uL 

Nasal smear 

None 

Malarial Smear 

Giemsa Stain 

Daily 

Six (6) hours 

3 cc whole blood / 500uL microtainer (EDTA) and two to three direct malarial smears (finger prick) 

Nasal smear 

Note the travel history of the patient and days of fever 

Silica Clotting Time (SCT) 

Absorbance 

Every Wednesday Cut-off: 12:00 NN 

Friday 

3cc citrated plasma 

None 

Centrifuge at 3500 rpm for 10 minutes separate plasma in a plastic tube, re-centrifuge and transfer plasma into a plastic tube (take care not to include the residual platelets that may have collected at the bottom of the centrifuge tube. Freeze sample immediately 

Lupus Anticoagulant Test 1 (SCT + DRVVT) 

See Individual Test 

Every Wednesday Cut-off: 12:00 NN 
Friday 
3cc citrated plasma 
None 

Prothrombin Time or Protime (PT) 

Absorbance 

Every Wednesday Cut-off: 12:00 NN 

Routine: Two (2) hours

STAT: One (1) hour 

3cc citrated plasma 

None 

Should be processed within 24 hours/immediately separate citrated plasma in a plastic tube, should there will be a delay in processing. Take note of patient's medication. 

PT Mixing Study 

Absorbance 
Every Wednesday Cut-off: 12:00 NN 

Four (4) hours 

3cc citrated plasma 
None 

Must be processed immediately 

Partial thromboplastin Time (PTT) Mixing Study 

Absorbance 
Every Wednesday Cut-off: 12:00 NN 
Four (4) hours 
3cc citrated plasma 
None 
Must be processed immediately 

Peripheral Smear 

Manual Method 

Daily 

Four hours 

Freshly collected, must be submitted to hematology within two hours after collection/3cc whole blood / 500uL microtainer (EDTA) or two direct smears (finger prick) 

None 

Mix by gentle inversion 8 to 10 times 

Peripheral Smear with Pathologist Consultation 

Manual Method 
Daily 

Refer to Hematology Section of Pathologist’s Availability 

Freshly collected, must be submitted to hematology within two hours after collection/3cc whole blood / 500uL microtainer (EDTA) or two direct smears (finger prick) 
None 
Mix by gentle inversion 8 to 10 times 

Platelet Count Estimation on Smear 

Manual Method 
Daily 

Routine: Two hours

STAT: One hour 

3 cc whole blood / 500uL microtainer (EDTA) 

None 
Mix by gentle inversion 8 to 10 times 

Reticulocyte Count, Manual 

Manual Method 
Daily 

Routine: Two hours

STAT: One hour 

3 cc whole blood / 500uL microtainer (EDTA) 
None 
Mix by gentle inversion 8 to 10 times 

Platelet Count 

Flow Cytometry 

Daily 

Routine: Two hours

STAT: One hour 

3 cc whole blood / 500uL microtainer (EDTA) 
None 
Mix by gentle inversion 8 to 10 times 

White Blood Cell (WBC) Count 

Flow Cytometry 
Daily 

Routine: Two hours

STAT: One hour 

3 cc whole blood / 500uL microtainer (EDTA) 
None 
Mix by gentle inversion 8 to 10 times 

WBC and Differential Count 

Flow Cytometry 

Daily 

Routine: Two (2) hours

STAT: One (1) hour 

3 cc whole blood / 500uL microtainer (EDTA) 

None 

Mix by gentle inversion 8 to 10 times