Acid fast stain, direct smear (afb stain)

Test Methodology:

Ziehl-Neelsen method


Running Day:

Daily


Turn Around Time:

Routine: 24 hours

STAT: 6 hours

For all samples received within Microbiology operating hours (7am-6pm)


Specimen Requirement:

Any specimen, collected in sterile container or in the form of a smear


Patient Preparation:

for sputum and urine specimens, early morning collection is ideal, preferably before breakfast. Rinse mouth with water prior to collection to avoid inclusion of food debris


Special Considerations:

Avoid exposing to sunlight as afb are easily destroyed by sunlight exposure


Unit Conducting the Service:

Microbiology